Translator Disclaimer
1 March 2005 THIDIAZURON-INDUCED HIGH-FREQUENCY SHOOT ORGANOGENESIS FROM LEAF-DERIVED CALLUS OF A MEDICINAL CLIMBER, TYLOPHORA INDICA (BURM. F.) MERRILL
Author Affiliations +
Abstract

Tylophora indica (Burm. f.) Merrill is a threatened medicinal climber distributed in the forests of northern and peninsular India. An efficient and reproducible protocol for high-frequency callus regeneration from immature leaf explants of T. indica was developed. Organogenic callus formation from immature leaf pieces was obtained by using Murashige and Skoog (MS) medium supplemented with 7 μM 2,4-dichlorophenoxyacetic acid and 1.5 μM 6-benzyladenine. On this medium 92% explants produced callus. The optimal hormone combination for plantlet regeneration was 8 μM thidiazuron, at which shoot regeneration was obtained from 100% of the cultures, with an average of 66.7 shoots per culture. Histological studies of the regenerative callus revealed that shoot buds were originated from the outermost regions. For root formation, half-strength MS medium supplemented with 3 μM indole-3-butyric acid was used. Plants were transferred to soil, where 92% survived after 3 mo. of acclimatization.

T. DENNIS THOMAS and BOBAN PHILIP "THIDIAZURON-INDUCED HIGH-FREQUENCY SHOOT ORGANOGENESIS FROM LEAF-DERIVED CALLUS OF A MEDICINAL CLIMBER, TYLOPHORA INDICA (BURM. F.) MERRILL," In Vitro Cellular and Developmental Biology - Plant 41(2), 124-128, (1 March 2005). https://doi.org/10.1079/IVP2004575
Received: 18 February 2004; Accepted: 1 June 2004; Published: 1 March 2005
JOURNAL ARTICLE
5 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top