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1 March 2005 EFFECT OF TRIACONTANOL ON THE MICROPROPAGATION OF COSTUS SPECIOSUS (KOEN.) SM. USING RHIZOME THIN SECTIONS
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Abstract

The highest percentage of shoot regeneration of Costus speciosus was achieved using thin rhizome sections and triacontanol (TRIA). Factors affecting the rate of shoot multiplication and rooting with TRIA have been investigated. Initiation of shoot buds was observed when rhizome thin sections were cultured on B5 basal medium supplemented with 5 μg l−1 TRIA. Shoots with two to three leaves produced roots when cultured on B5 basal medium supplemented with 2 μg l−1 TRIA. The well-rooted shoots were hardened and transferred to soil where they showed normal growth and a 100% survival rate was achieved. Results of this study showed that TRIA can be used as an effective growth regulator in the micropropagation and conservation of C. speciosus.

RAVINDRA B. MALABADI, GANGADHAR S. MULGUND, and K. NATARAJA "EFFECT OF TRIACONTANOL ON THE MICROPROPAGATION OF COSTUS SPECIOSUS (KOEN.) SM. USING RHIZOME THIN SECTIONS," In Vitro Cellular and Developmental Biology - Plant 41(2), 129-132, (1 March 2005). https://doi.org/10.1079/IVP2004586
Received: 26 January 2004; Accepted: 1 July 2004; Published: 1 March 2005
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