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1 March 2005 DIRECT PLANT REGENERATION OF CURRY LEAF TREE (MURRAYA KOENIGII KOENIG.), AN AROMATIC PLANT
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Abstract

An efficient protocol for plant regeneration from stem segments of Murraya koenigii was developed by culturing on Murashige and Skoog (MS) medium supplemented with 2.5 mg l−1 benzyladenine (BA), 25 mg l−1 adenine sulfate, 0.25 mg l−1 indole-3-acetic acid (IAA), and 3% sucrose. The frequency of shoot bud regeneration was higher on similar medium in subsequent subcultures. The regenerated shoots were rooted on half-strength basal MS medium supplemented with 0.25–0.5 mg l−1 IAA or 1-naphthaleneacetic acid (NAA) within 8–12 d of culture. The maximum percentage of rooting was obtained on MS medium supplemented with IAA and NAA, each at 0.25 mg l−1. During acclimatization, 95% of rooted plantlets survived and were grown normally under greenhouse conditions.

GYANA RANJAN ROUT "DIRECT PLANT REGENERATION OF CURRY LEAF TREE (MURRAYA KOENIGII KOENIG.), AN AROMATIC PLANT," In Vitro Cellular and Developmental Biology - Plant 41(2), 133-136, (1 March 2005). https://doi.org/10.1079/IVP2004613
Received: 23 April 2004; Accepted: 1 October 2004; Published: 1 March 2005
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