The effects of growth regulators on culture response of different pyrethrum (Chrysanthemum cinerariaefolium Vis.) genotypes were investigated. In the genotype Sb/66/107, the presence of 2,4-dichorophenoxyacetic acid (2,4-D) at 2 mg l⁻¹ promoted growth of callus, whereas benzyladenine and 1-naphthaleneacetic acid had no effect. Callus growth was also affected by the 2,4-D concentration and genotypes. The optimal callus growth for the nine pyrethrum genotypes varied widely in response to 2,4-D, ranging from 0.5 mg l⁻¹ for genotype Marwanga to 3.0 mg l⁻¹ for Ks/75/336. Among the genotypes, shoots were regenerated from calluses of Sb/66/107, 4331, Marwanga, and MA/70/1013.