ROLE OF ANTIOXIDANTS AND AMINO ACIDS ON SOMATIC EMBRYOGENESIS OF PINUS PATULA

RAVINDRA B. MALABADI; JOHANNES VAN STADEN

doi:10.1079/IVP2004623

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RAVINDRA B. MALABADI,¹ JOHANNES VAN STADEN^1,2

¹Research Centre for Plant Growth and Development, School of Botany and Zoology, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa
²Author to whom correspondence should be addressed: vanstadenj@ukzn.ac.za

Abstract

This study investigated the role of antioxidants and amino acids on somatic embryogenesis using the vegetative shoot apices of three genotypes of mature Pinus patula trees. Apart from dithiothreitol at 0.1%, pretreatment of explants or incorporation of antioxidants in the basal nutrient medium had a negative effect on the initiation of embryogenic cultures, somatic embryo production, and plantlet recovery. Inclusion of an amino acid solution mixture, at a concentration of 10.0 mM, in the maturation medium tended to increase somatic embryo production.

Citation Download Citation

RAVINDRA B. MALABADI and JOHANNES VAN STADEN "ROLE OF ANTIOXIDANTS AND AMINO ACIDS ON SOMATIC EMBRYOGENESIS OF PINUS PATULA," In Vitro Cellular and Developmental Biology - Plant 41(2), 181-186, (1 March 2005). https://doi.org/10.1079/IVP2004623

Received: 13 August 2004; Accepted: 1 November 2004; Published: 1 March 2005