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1 July 2005 PHOTOSYNTHESIS AND CARBON METABOLISM IN PLANTAIN (MUSA AAB) PLANTLETS GROWING IN TEMPORARY IMMERSION BIOREACTORS AND DURING EX VITRO ACCLIMATIZATION
CARLOS E. ARAGÓN, MARITZA ESCALONA, IRIS CAPOTE, DANILO PINA, INAUDIS CEJAS, ROBERTO RODRIGUEZ, MARIA JESUS CAÑAL, JORGE SANDOVAL, SOPHIE ROELS, PIERRE DEBERGH, JUSTO GONZALEZ-OLMEDO
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Abstract

The photosynthetic capacity changes and the main enzymatic systems related to carbon metabolism were investigated during the in vitro culture of plantain shoots (Musa AAB cv. CEMSA 3/4) in temporary immersion bioreactors (TIB) and their subsequent acclimatization. The maximal rate of photosynthesis (Pn), transpiration, and the activity of the carbon metabolism enzymes phosphoenolpyruvate carboxylase (PEPC), acid invertase (AI), pyruvate kinase (PK) and sucrose phosphate synthase (SPS) were measured every 7 d during the 21 d of elongation in TIB, and the following 42 d of acclimatization. Sucrose content in the liquid medium and in the leaves was also determined. The most significant changes in plant growth were observed during acclimatization. During the in vitro stage photosynthesis was limited (4–6 μmol CO2 m−2 s−1); the photosynthetic rate however increases rapidly and significantly as soon as in vitro culture is over during acclimatization. PEPC activity increased during the whole evaluation period. The highest levels were achieved around days 42 and 56. PK and SPS activities were optimal during the first weeks in acclimatization (28–35 d), while AI increased at the beginning of the elongation phase (7 d), and later at the end of the acclimatization (49–63 d). The relationships between morphological parameters, photosynthetic capacity of the plantlets and the carbon metabolism enzymes during both phases of the culture are discussed.

CARLOS E. ARAGÓN, MARITZA ESCALONA, IRIS CAPOTE, DANILO PINA, INAUDIS CEJAS, ROBERTO RODRIGUEZ, MARIA JESUS CAÑAL, JORGE SANDOVAL, SOPHIE ROELS, PIERRE DEBERGH, and JUSTO GONZALEZ-OLMEDO "PHOTOSYNTHESIS AND CARBON METABOLISM IN PLANTAIN (MUSA AAB) PLANTLETS GROWING IN TEMPORARY IMMERSION BIOREACTORS AND DURING EX VITRO ACCLIMATIZATION," In Vitro Cellular and Developmental Biology - Plant 41(4), 550-554, (1 July 2005). https://doi.org/10.1079/IVP2005640
Received: 25 June 2004; Accepted: 1 February 2005; Published: 1 July 2005
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