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1 July 2005 EFFECTS OF GENOTYPE, EXPLANT ORIENTATION, AND WOUNDING ON SHOOT REGENERATION IN TOMATO
POONAM BHATIA, NANJAPPA ASHWATh, DAVID J. MIDMORE
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Abstract

Effects of genotype and explant orientation on shoot regeneration from cotyledonary explants of tomato were studied using 10 commercially important cultivars. The explant orientation affected shoot regeneration in all the tested genotypes. Cotyledons placed in abaxial (lower surface facing down) orientation consistently produced better shoot regenerative response and produced greater numbers and taller shoots compared to those inoculated in adaxial (upper surface facing down) orientation. Genotypic variation in terms of shoot regeneration response, number of shoots, and shoot height was apparent. Wounding of cotyledonary explants increased shoot regeneration response. However, shoot height was much lower in shoots regenerated from wounded explants compared to those that originated from intact cotyledons. Shoots produced from wounded cotyledons were abnormal in their form and structure.

POONAM BHATIA, NANJAPPA ASHWATh, and DAVID J. MIDMORE "EFFECTS OF GENOTYPE, EXPLANT ORIENTATION, AND WOUNDING ON SHOOT REGENERATION IN TOMATO," In Vitro Cellular and Developmental Biology - Plant 41(4), 457-464, (1 July 2005). https://doi.org/10.1079/IVP2005649
Received: 13 July 2004; Accepted: 1 February 2005; Published: 1 July 2005
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