We report a protocol for somatic embryogenesis and plantlet regeneration of Buchanania lanzan Spreng (Family – Anacardiaceae), which is a tropical fruit tree widely distributed in the dry forests of India. Calluses were initiated from immature zygotic embryos cultured on Murashige and Skoog (MS) medium supplemented with various combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzyladenine (BA) and/or 1-naphthaleneacetic acid (NAA). The highest frequency (60%) of somatic embryo induction was obtained in cultures grown on MS medium fortified with 4.53 μM 2,4-D, 5.32 μM NAA and 4.48 μM BA. The medium supplemented with 15 μM abscisic acid (ABA) was most effective for maturation and germination of somatic embryos. This is the first report on somatic embryogenesis in B. lanzan, which may be helpful for in vitro propagation, ex situ conservation and genetic manipulation of this species.
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1 September 2005
SOMATIC EMBRYOGENESIS IN BUCHANANIA LANZAN SPRENG
P. SHARMA,
VIJAYA KOCHE,
AFAQUE QURAISHI,
S. K. MISHRA
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In Vitro Cellular and Developmental Biology - Plant
Vol. 41 • No. 5
September 2005
Vol. 41 • No. 5
September 2005
Abscisic acid
Adenine sulfate
chironji
differentiation
in vitro
Zygotic embryo