A simple and effective procedure has been developed for plantlet regeneration from cotyledon-derived callus of the medicinally important herb and ornamental species, Incarvillea sinensis. An average of 18.4 adventitious shoots per explant were obtained from 100% cotyledon explants cultured on half-strength Murashige and Skoog (MS) medium containing 1.0 mg l−1 6-benzylaminopurine for 3 wk, followed by another 4 wk on hormone-free 1/2×MS medium. The cotyledon explants continued to expand and regenerate new shoots upon repeated subculturing onto fresh medium. Most regenerated shoots (66.9%) were rooted on 1/4×MS medium containing 1.0 mg l−1 indole-3-acetic acid, with an average of about 3.8 roots per shoot. Regenerated plants with well developed shoots and roots were successfully acclimatized in soil and were normal phenotypically.
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1 September 2005
HIGH EFFICIENCY IN VITRO PLANT REGENERATION FROM COTYLEDON EXPLANTS OF INCARVILLEA SINENSIS
ZHONG HU,
WEI LI,
GUANG-QIN GUO
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In Vitro Cellular and Developmental Biology - Plant
Vol. 41 • No. 5
September 2005
Vol. 41 • No. 5
September 2005
adventitious shoots
Callus
Incarvillea sinensis Lam
Tissue culture