An efficient plant regeneration protocol for rapidly propagating Rhodiola fastigiata (Hk. f. et Thoms.) S.H.FU, a traditional Chinese medicinal plant, was developed. Shoot organogenesis occurred from the leaf explants inoculated on medium with appropriate supplements of plant growth regulators. Up to 5.3 shoots formed per leaf explant cultured on a medium containing 13.32 μM 6-benzylaminopurine (BA) and 0.54 μM 1-naphthaleneacetic acid (NAA). Regenerated shoots formed complete plantlets on a medium containing 1.48 μM indole-3-butyric acid (IBA), and mature plants were established, acclimatized, and thrived in greenhouse conditions. The regeneration protocol developed in this study provides a basis for germplasm conservation and for further investigation of medicinally active constituents of the elite Chinese medicinal plant.
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1 July 2006
PLANT REGENERATION FROM LEAF EXPLANTS OF RHODIOLA FASTIGIATA
HAI-JUN LIU,
YAN XU,
YU-JUN LIU,
CHUN-ZHAO LIU
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In Vitro Cellular and Developmental Biology - Plant
Vol. 42 • No. 4
July 2006
Vol. 42 • No. 4
July 2006
Chinese traditional medicinal plant
leaf explant
Rhodiola fastigiata
shoot organogenesis