This study reports the direct regeneration of flower buds from cultured sepal segments of Sinningia speciosa Hiern. Two types of floral bud regeneration were observed: regeneration of floral buds only (designed as B^F) and regeneration of both floral and vegetative buds (designed as B^{F V}). The capacity of B^F regeneration was closely related to the location of sepal segments and the concentration of exogenous gibberellin (GA₃) and cytokinin in the medium. On the medium containing 1.0 mg l⁻¹ GA₃, the addition of 6-benzyladenine (BA) significantly increased the frequency of total flower bud (B^F B^{F V}) formation, with the frequency up to 91.5% in the presence of 0.4 mg l⁻¹ BA. On the medium containing 0.1 mg l⁻¹ BA, the addition of GA₃ also increased the frequency of total flower bud regeneration, with the frequency up to 74.3% in the presence of 1.0 mg l⁻¹ GA₃, but no further increase in regeneration was observed when the GA₃ concentration was higher than 1.0 mg l⁻¹. The capacity of B^F regeneration from different locations of sepal segments was differential. The adaxial part of sepal segments gave rise to the highest frequency of 56.7 and 84.3% for B^F and B^F B^{F V}, respectively.