Cotyledonary node and leaf nodal explants excised from 14-d-old in vitro-grown seedlings of Albizia odoratissima were cultured on a Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP), N6-(2-isopentenyl) adenine (2-iP) and kinetin, used either solely or in combinations. The highest frequency for shoot regeneration (82.5%), the maximum number of shoots per explant (6.9), and the maximum shoot length (2.55 cm) were obtained from cotyledonary node explants cultured on a MS medium containing 10 μM BAP and 10 μM 2-iP with 30 g l−1 sucrose. Successful rooting was achieved by placing the microshoots on MS medium with 25 μM indole-3-butyric acid (IBA) for 24 h first, then transferring to the same medium without IBA. Of the various substrates tested, vermiculite was best for plant acclimatization, in which 75% of plants survived.
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1 September 2006
IN VITRO PROPAGATION OF ALBIZIA ODORATISSIMA L.F. (BENTH.) FROM COTYLEDONARY NODE AND LEAF NODAL EXPLANTS
V. RAJESWARI,
KAILASH PALIWAL
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In Vitro Cellular and Developmental Biology - Plant
Vol. 42 • No. 5
September 2006
Vol. 42 • No. 5
September 2006
black siris
In vitro rooting
Indole-3-butyric acid
Plant regeneration
Shoot multiplication