Dendrobium hybrids have great economic importance in a number of countries. Asymbiotic seed germination and the conventional vegetative method have been commonly used by growers to propagate these plants. To overcome somaclonal variation, which is commonly exhibited by Dendrobium (Nobile group) when micropropagated from protocorm-like bodies, a protocol for propagating Dendrobium Second Love in vitro using axillary buds in the presence of thidiazuron was developed. Random amplified polymorphic DNA analysis was also carried out to check for possible genetic alterations in plants originating from six consecutive subcultures. The results revealed that the established protocol was efficient for the in vitro cloning of this orchid hybrid and the plants obtained from the six subcultures did not exhibit any type of polymorphism.
How to translate text using browser tools
1 November 2006
MICROPROPAGATION AND GENETIC STABILITY OF A DENDROBIUM HYBRID (ORCHIDACEAE)
WAGNER DE MELO FERREIRA,
GILBERTO BARBANTE KERBAUY,
ANA PAULA PIMENTEL COSTA
ACCESS THE FULL ARTICLE
It is not available for individual sale.
This article is only available to subscribers.
It is not available for individual sale.
It is not available for individual sale.
In Vitro Cellular and Developmental Biology - Plant
Vol. 42 • No. 6
November 2006
Vol. 42 • No. 6
November 2006
In vitro propagation
orchid
random amplified polymorphic DNA (RAPD)
Thidiazuron