Translator Disclaimer
1 November 2006 IN VITRO PROPAGATION OF CLEOME SPINOSA (CAPPARACEAE) USING EXPLANTS FROM NURSERY-GROWN SEEDLINGS AND AXENIC PLANTS
NORMA ALBARELLO, CLAUDIA SIMÕES, PAULA FARIA GONÇALVES ROSAS, TATIANA CARVALHO DE CASTRO, MÁRCIA GARCIA GIANFALDONI, CÁTIA HENRIQUES CALLADO, ELISABETH MANSUR
Author Affiliations +
Abstract

Two independent experiments were performed to establish micropropagation of Cleome spinosa from stem segments. In the first experiment, direct shoot organogenesis on hypocotyl explants from 2-mo.-old nursery-grown seedlings was obtained on Murashige and Skoog medium with different combinations of benzyladenine (BA) and 6-furfurylaminopurine, added either individually or in combination. Best proliferation rates occurred in the presence of 2.2 and 4.4 μM BA and the highest mean number of shoots was produced in response to 4.4 μM BA. In the second experiment, regeneration via direct organogenesis was also obtained from nodal and internodal segments of axenic plants cultured in the presence of BA (4.4 and 8.8 μM) in association with indole-3-acetic acid (IAA) (0.57 and 1.14 μM). Internodal explants were the most responsive on all media tested. The best mean number of shoots per explant was achieved on medium with 4.4 μM BA in association with 0.57 μM IAA. Histological studies of the globular structures formed at the apical portion of the explants revealed direct shoot regeneration and adventitious shoot differentiation from meristematic centers around the vascular bundles of the primary regenerants. All shoots elongated and rooted on MS0 medium. The acclimatization rates ranged between 70 and 84%. Plants reached to maturity and flowered 4 mo. after transfer to ex vitro conditions.

NORMA ALBARELLO, CLAUDIA SIMÕES, PAULA FARIA GONÇALVES ROSAS, TATIANA CARVALHO DE CASTRO, MÁRCIA GARCIA GIANFALDONI, CÁTIA HENRIQUES CALLADO, and ELISABETH MANSUR "IN VITRO PROPAGATION OF CLEOME SPINOSA (CAPPARACEAE) USING EXPLANTS FROM NURSERY-GROWN SEEDLINGS AND AXENIC PLANTS," In Vitro Cellular and Developmental Biology - Plant 42(6), 601-606, (1 November 2006). https://doi.org/10.1079/IVP2006828
Received: 30 August 2005; Accepted: 10 September 2006; Published: 1 November 2006
JOURNAL ARTICLE
6 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top