Up to 73% decrease in cost of media for plant regeneration and in vitro conservation was achieved in Curcuma longa cv Prathibha by using inexpensive carbon source and gelling agent. Laboratory reagent-grade sucrose was replaced by locally available commercial sugar (market sugar or sugar cubes) as carbon source and bacteriological grade agar by isabgol (also named isubgol) as gelling agent. No adverse effects on shoot regeneration and conservation on isabgol-gelled low-cost media were observed as compared to that on agar-gelled control medium (CM). Some 33–56% cultures of C. longa survived up to 12 mo. on isabgol-gelled medium in comparison to only 16% on CM. Genetic stability of 12-month-old in vitro-conserved plants was assessed using 25 random amplified polymorphic DNA (RAPD) primers; no significant variation was observed in RAPD profiles of mother plants and in vitro-conserved plantlets on CM and low-cost media.
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9 February 2007
Low-cost media for in vitro conservation of turmeric (Curcuma longa L.) and genetic stability assessment using RAPD markers
Rishi K. Tyagi,
Anuradha Agrawal,
C. Mahalakshmi,
Zakir Hussain,
Husnara Tyagi
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In Vitro Cellular and Developmental Biology - Plant
Vol. 43 • No. 1
February 2007
Vol. 43 • No. 1
February 2007
Curcuma
Isabgol
Isubgol
Low-cost tissue culture media
matric potential
osmotic potential
Psyllium husk