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16 March 2007 Micropropagation of Penthorum chinense through axillary bud
H. Cao, J. Yang, Z. S. Peng, C. Y. Kang, D. C. Chen, Z. C. Gong, X. Tan
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Abstract

This study reports a protocol for successful micropropagation of Penthorum chinense using nodal explants on Murashige and Skoog (MS) medium supplemented with 6-benzyladenine (BA) or kinetin (Kn). The presence of BA promoted a higher rate of shoot multiplication than Kn. Maximum multiple shoot formation was observed in 59.2% of nodal explants cultured on MS medium supplemented with 2.0 mg l−1 BA after 6 wk. After subculture for 4 wk, the maximum number of shoots (6.4) was obtained on a medium with 2.0 mg l−1 BA, but shoots were too short and not suitable for micropropagation. The taller shoots that regenerated in the presence of lower BA concentration (1.0 mg l−1) were selected for root induction study. Most shoots (98.8%) rooted in the presence of 0.5 mg l−1 indole-3-acetic acid after 3 wk, with each shoot forming an average of 10.0 roots. Plantlets were transferred to soil and successfully acclimatized.

H. Cao, J. Yang, Z. S. Peng, C. Y. Kang, D. C. Chen, Z. C. Gong, and X. Tan "Micropropagation of Penthorum chinense through axillary bud," In Vitro Cellular and Developmental Biology - Plant 43(2), 149-153, (16 March 2007). https://doi.org/10.1007/s11627-007-9034-9
Received: 8 March 2006; Accepted: 22 September 2006; Published: 16 March 2007
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KEYWORDS
In vitro propagation
Medicinal plant
Penthorum chinense
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