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16 March 2007 In vitro flowering of Perilla frutescens
T. Zhang
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Abstract

This study investigated the factors affecting in vitro flowering of Perilla frutescens. The shoots regenerated from cotyledonary and hypocotyl explants cultured on Murashige and Skoog (MS) medium supplemented with benzyladenine (BA) and indole-3-acetic acid, each at 0.5 mg l−1, were excised and transferred to MS medium containing 30 g l−1 of sucrose, 8.25 g l−1 of ammonium nitrate, and 1.0 mg l−1 of BA. After 40 d of culture, 86.2% of shoots flowered and most of which self-fertilized in vitro and produced mature fruits with viable seeds. These seeds were germinated and plants were grown to maturity and flowered in soil under greenhouse conditions. The in vitro flowering system reported in this study may facilitate rapid breeding of P. frutescens and offers a model system for studying the physiological mechanism of flowering.

T. Zhang "In vitro flowering of Perilla frutescens," In Vitro Cellular and Developmental Biology - Plant 43(2), 91-94, (16 March 2007). https://doi.org/10.1007/s11627-007-9038-5
Received: 9 June 2006; Accepted: 13 November 2006; Published: 16 March 2007
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KEYWORDS
fruiting
In vitro flowering
Perilla frutescens
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