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18 April 2007 Direct somatic embryogenesis from cotyledon and cotyledonary node explants in bishop's weed Trachyspermum ammi (L.) sprague
Smita Purohit, S. L. Kothari
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Abstract

A three-stage procedure for embryogenesis in Trachyspermum ammi was developed from cotyledon and cotyledonary node explants cultured in Murashige and Skoog (MS) liquid medium supplemented with 0.2 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D). Globular somatic embryos without intervening callus phase developed in 4 wk. The development of embryos to heart and torpedo stages required second-stage subculture of the explants (along with developing embryos) in liquid medium with lower concentrations of 2,4-D. Further development of embryos required a third-stage subculture in hormone-free liquid medium supplemented with 100 mg l−1 casein hydrolysate. Regeneration of complete plantlets occurred after the fully developed somatic embryos were transferred to solidified half-strength MS medium supplemented with 1 mg l−1 gibberellic acid.

Smita Purohit and S. L. Kothari "Direct somatic embryogenesis from cotyledon and cotyledonary node explants in bishop's weed Trachyspermum ammi (L.) sprague," In Vitro Cellular and Developmental Biology - Plant 43(2), 154-158, (18 April 2007). https://doi.org/10.1007/s11627-007-9039-4
Received: 6 June 2006; Accepted: 1 November 2006; Published: 18 April 2007
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KEYWORDS
Apiaceae
Carum copticum
regeneration
Somatic embryos
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