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25 April 2007 Rapid plant regeneration through in vitro axillary shoot proliferation of butterfly pea (Clitoria ternatea L.)—a twinning legume
D. P. Barik, S. K. Naik, A. Mudgal, P. K. Chand
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Abstract

This study describes a reproducible protocol for rapid mass propagation of a multipurpose legume, Clitoria ternatea L., using cotyledonary node explants derived from axenic seedlings. Multiple shoots were induced in Murashige and Skoog (MS) medium supplemented with N 6-benzyladenine (BA), zeatin riboside, or thidiazuron. N 6-Benzyladenine at 1.0 mg l−1 (4.44 μM) was most effective for shoot proliferation. Multiple shoots were also induced in nodal segments of in vitro-raised shoots grown on MS medium containing 1.0 mg l−1 (4.44 μM) of BA. Rooting was best induced in shoots grown on half-strength MS medium with 0.25 mg l−1 (1.42 μM) of indole-3-butyric acid. Plants were acclimatized in vermicompost and established in soil where they flowered and formed mature seeds.

D. P. Barik, S. K. Naik, A. Mudgal, and P. K. Chand "Rapid plant regeneration through in vitro axillary shoot proliferation of butterfly pea (Clitoria ternatea L.)—a twinning legume," In Vitro Cellular and Developmental Biology - Plant 43(2), 144-148, (25 April 2007). https://doi.org/10.1007/s11627-007-9040-y
Received: 20 February 2006; Accepted: 7 August 2006; Published: 25 April 2007
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KEYWORDS
Butterfly pea
Cotyledonary node
Growth regulators
micropropagation
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