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29 March 2007 Rapid clonal propagation of Curculigo orchioides Gaertn., an endangered medicinal plant
Salema Valencio Francis, Sunil Kumar Senapati, Gyana Ranjan Rout
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Abstract

An efficient protocol was developed for in vitro clonal propagation of Curculigo orchioides Gaertn. through apical meristem culture. Multiple shoots were induced from apical meristems grown on Murashige and Skoog (MS) basal medium supplemented with 1.5 mg l−1 6-benzyladenine (BA), 100 mg l−1 adenine sulfate (Ads) and 3% sucrose. Inclusion of indole-3-butyric acid (IBA) or indole-3-acetic acid (IAA) in the culture medium improved the formation of multiple shoots. The highest frequency of multiplication was obtained on MS medium supplemented with 1.5 mg l−1 BA, 100 mg l−1 Ads, 0.25 mg l−1 IBA and 3% sucrose. Rooting was achieved upon transferring the micro-shoots to half-strength MS medium containing 0.25 mg l−1 IBA and 2% sucrose. Micropropagtated plantlets were hardened in the greenhouse and successfully established in soil.

Salema Valencio Francis, Sunil Kumar Senapati, and Gyana Ranjan Rout "Rapid clonal propagation of Curculigo orchioides Gaertn., an endangered medicinal plant," In Vitro Cellular and Developmental Biology - Plant 43(2), 140-143, (29 March 2007). https://doi.org/10.1007/s11627-007-9041-x
Received: 9 December 2005; Accepted: 7 August 2006; Published: 29 March 2007
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KEYWORDS
Anticancer properties
Growth regulators
medicinal plants
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