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21 February 2007 High frequency plantlet regeneration from cotyledonary node cultures of Aegle marmelos (L.) Corr
Pranati Nayak, P. R. Behera, Thirunavoukkarasu Manikkannan
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Abstract

High frequency plantlet regeneration was achieved in cotyledonary nodes of Aegle marmelos. Cotyledonary nodes from 1 mo. old in vitro grown seedlings of A. marmelos were cultured on Murashige and Skoog (MS) medium supplemented with benzyl adenine (BA) (0–8.8 μM), kinetin (KIN) (0–9.4 μM), and indole-3-acetic acid (IAA) (0–1.14 μM) either alone or in combinations. The highest regenerative response was observed on medium containing 6.6 μM BA 1.14 μM IAA where approximately 86.6% of the cultures responded with an average shoot numbers of 487.5 per explant in 7-wk time. Cultures maintained on KIN-supplemented medium showed very poor response. In vitro responded shoots were transferred to root induction medium consisting of half-strength MS supplemented with auxins IAA, indole-3-butyric acid (IBA), or α-naphthalene acetic acid (NAA). Rooting was best in medium supplemented with 14.7 μM IBA. Rooted plantlets were acclimatized and transferred to the field with 80% survival rate.

Pranati Nayak, P. R. Behera, and Thirunavoukkarasu Manikkannan "High frequency plantlet regeneration from cotyledonary node cultures of Aegle marmelos (L.) Corr," In Vitro Cellular and Developmental Biology - Plant 43(3), 231-236, (21 February 2007). https://doi.org/10.1007/s11627-006-9013-6
Received: 1 December 2006; Accepted: 1 December 2006; Published: 21 February 2007
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