Translator Disclaimer
5 May 2007 Clonal propagation of mesquite tree (Prosopis laevigata Humb. & Bonpl. ex Willd. M.C. Johnston). I. Via cotyledonary nodes
Author Affiliations +
Abstract

Plantlet regeneration in Prosopis laevigata (Humb. & Bonpl. ex Willd.) Johnston (Fabaceae), a multipurpose tree, has been achieved from cotyledonary nodes excised from in vitro grown seedlings. The explants were cultured on MS media containing different concentrations of N-6 benzyladenine (BA) and 2,4-dichlorophenoxyacetic acid (2,4-d) and a mixture of organic components. The highest number (3.37 0.51) of multiple shoots was observed in MS media containing 2,4-d (9.05 μM) BA (6.62 μM). The regenerated shoots were then transferred onto half-strength MS medium containing a plant growth regulator that was either: indole-3-butyric acid, 1-naphthaleneacetic, indole-3-acetic acid, or 2,4-d as well as phytagel or vermiculite for adventitious root initiation. Best rooting efficiency of 44.0% was obtained when NAA (16.11 μM) and vermiculite were used. After rooting, the cloned plantlets were successfully hardened to ex vitro conditions. This work may help to reduce the devastation caused by the overexploitation of this species.

L. Buendía-González, J. Orozco-Villafuerte, F. Cruz-Sosa, V. M. Chávez-Ávila, E. J. Vernon-Carter, and R. J. Newton "Clonal propagation of mesquite tree (Prosopis laevigata Humb. & Bonpl. ex Willd. M.C. Johnston). I. Via cotyledonary nodes," In Vitro Cellular and Developmental Biology - Plant 43(3), 260-266, (5 May 2007). https://doi.org/10.1007/s11627-007-9027-8
Received: 29 September 2006; Accepted: 1 January 2007; Published: 5 May 2007
JOURNAL ARTICLE
7 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top