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18 October 2007 Micropropagation and induction of autotetraploid plants of Chrysanthemum cinerariifolium (Trev.) Vis
Zhen Liu, Shanlin Gao
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Abstract

Rapid propagation technology was established and optimized in vitro for Chrysanthemum cinerariifolium (Trev.) Vis., an important botanical insecticide plant with a huge international market. A large number of buds could be induced directly from epicotyl and hypocotyl explants on Murashige T; Skoog F. J. Plant. Physiol. 15: 473–479; (1962) medium [Murashige and Skoog (MS) medium] supplemented with 0.3 mg l−1 benzyladenine (BA) and 0.3 mg l−1 α-naphthaleneacetic acid (NAA). Root induction and development could be observed within 15 d after inoculation on 1/2 MS medium supplemented with 0.2 mg l−1 indole-3-acetic acid (IAA) and 0.1 mg l−1 rooting powder (ABT). Furthermore, a polyploid breeding study in vitro was reported to obtain superior breeding lines with high yield and good quality. Autotetraploid lines of C. cinerariifolium were obtained by colchicine treatments and identified by root-tip chromosome determination and stoma observation. The chromosome number of the autotetraploid plantlet was 2N = 4x = 36. Obtained autotetraploid lines will be of important genetic and breeding value and be used for further selection and plant breeding.

Zhen Liu and Shanlin Gao "Micropropagation and induction of autotetraploid plants of Chrysanthemum cinerariifolium (Trev.) Vis," In Vitro Cellular and Developmental Biology - Plant 43(5), 404-408, (18 October 2007). https://doi.org/10.1007/s11627-007-9085-y
Received: 30 August 2006; Accepted: 29 August 2007; Published: 18 October 2007
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