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31 August 2007 Somatic embryogenesis in Agave tequilana Weber cultivar azul
Liberato Portillo, Fernando Santacruz-Ruvalcaba, Antonia Gutiérrez-Mora, Benjamín Rodríguez-Garay
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Abstract

Somatic embryogenesis was achieved from leaves of Agave tequilana Weber cultivar azul utilizing MS medium supplemented with L2 vitamins and the addition of cytokinins: 6-benzylaminopurine (BA), 1-phenyl-3(1,2,3-thiadiazol-5-yl)urea (TDZ), 6-(γ-γ-dimethylamino)purine (2ip) and 6-furfurylaminopurine (KIN), combined with the auxin 2,4-dichlorophenoxyacetic acid (2,4-D). Differences among the six genotypes studied with regard to their embryogenic response in culture were found. Embryos produced by genotype S3 under a hormone regime of high cytokinin (44.4 to 66.6 μM BA) compared to auxin (4.5 μM 2,4-D) contained chlorophyll, whereas those produced when auxin was high compared to cytokinin (9.0 and 13.6 μM 2,4-D and 1.3 and 4.0 μM BA, respectively) were whitish and morphologically similar to their zygotic counterparts. Somatic embryos matured and germinated after transferring the embryogenic calli to maturation and germination medium without growth regulators and enriched with organic nitrogen. Microscopic observations demonstrated a unicellular origin for production of indirect somatic embryos.

Liberato Portillo, Fernando Santacruz-Ruvalcaba, Antonia Gutiérrez-Mora, and Benjamín Rodríguez-Garay "Somatic embryogenesis in Agave tequilana Weber cultivar azul," In Vitro Cellular and Developmental Biology - Plant 43(6), 569-575, (31 August 2007). https://doi.org/10.1007/s11627-007-9046-5
Received: 17 December 2006; Accepted: 10 April 2007; Published: 31 August 2007
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