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19 August 2007 Interactive effect of light, temperature and TDZ on the regeneration potential of leaf discs of Fragaria x ananassa Duch
Amjad M. Husaini, M. Z. Abdin
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Abstract

This is the first report where shoot regeneration in strawberry cultivar Chandler has been achieved simultaneously through both somatic embryogenesis and shoot bud formation. Direct somatic embryogenesis was observed in leaf discs which were cultured on medium containing MS salts B5 vitamins 2% glucose 18.16 μM thidiazuron (TDZ) and given both chilling and dark treatment for 2 wk at 4±2°C followed by incubation at 25±2°C under 16-h photoperiod for third wk. After 3 wk, these explants were then subcultured on medium containing MS salts B5 vitamins 2% glucose and incubated under 16-h photoperiod at 25±2°C for further growth and development. Direct regeneration via de novo shoot bud formation was observed in leaf disks which were given dark treatment and were cultured on medium containing MS salts B5 vitamins 2% glucose supplemented with 9.08 μM TDZ. There was a synergistic effect of photoperiod, dark, and chilling treatments on somatic embryogenesis, whereas chilling treatment had an inhibitory effect on shoot organogenesis.

Amjad M. Husaini and M. Z. Abdin "Interactive effect of light, temperature and TDZ on the regeneration potential of leaf discs of Fragaria x ananassa Duch," In Vitro Cellular and Developmental Biology - Plant 43(6), 576-584, (19 August 2007). https://doi.org/10.1007/s11627-007-9048-3
Received: 6 October 2006; Accepted: 15 February 2007; Published: 19 August 2007
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