Disarming and sequencing of Agrobacterium rhizogenes strain K599 (NCPPB2659) plasmid pRi2659

S. Luke Mankin; D. Steven Hill; Paula M. Olhoft; Effie Toren; Allan R. Wenck; Lawrence Nea; Liqun Xing; Jeffrey A. Brown; Huihua Fu; Lesley Ireland; Hongmei Jia; Helke Hillebrand; Todd Jones; Hee-Sook Song; P. Ozias-Akins

doi:10.1007/s11627-007-9071-4

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19 September 2007 Disarming and sequencing of Agrobacterium rhizogenes strain K599 (NCPPB2659) plasmid pRi2659

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In Vitro Cellular and Developmental Biology - Plant, 43(6):521-535 (2007). https://doi.org/10.1007/s11627-007-9071-4

Abstract

Agrobacterium rhizogenes strain K599 (pRi2659), a causative agent of hairy root disease, effectively induces hairy root formation in a variety of plant species, including numerous soybean (Glycine max) cultivars. Because Agrobacterium-mediated transformation of soybean remains challenging and labor intensive, K599 appeared a suitable progenitor for new agrobacteria strains for plant transformation. In this paper, we report the disarming and sequencing of pRi2659 and the usefulness of the resulting disarmed strain in plant transformation studies of Arabidopsis thaliana, maize (Zea mays), tomato (Lycopersicon esculentum), and soybean (G. max).

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S. Luke Mankin, D. Steven Hill, Paula M. Olhoft, Effie Toren, Allan R. Wenck, Lawrence Nea, Liqun Xing, Jeffrey A. Brown, Huihua Fu, Lesley Ireland, Hongmei Jia, Helke Hillebrand, Todd Jones, Hee-Sook Song, and P. Ozias-Akins "Disarming and sequencing of Agrobacterium rhizogenes strain K599 (NCPPB2659) plasmid pRi2659," In Vitro Cellular and Developmental Biology - Plant 43(6), 521-535, (19 September 2007). https://doi.org/10.1007/s11627-007-9071-4

Received: 8 November 2006; Accepted: 1 July 2007; Published: 19 September 2007

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