Arginine and ornithine decarboxylases in embryogenic and non-embryogenic carrot cell suspensions

Natalia Loukanina; Trevor A. Thorpe

doi:10.1007/s11627-007-9080-3

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19 September 2007 Arginine and ornithine decarboxylases in embryogenic and non-embryogenic carrot cell suspensions

Natalia Loukanina, Trevor A. Thorpe

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In Vitro Cellular and Developmental Biology - Plant, 44(1):59-64 (2007). https://doi.org/10.1007/s11627-007-9080-3

Abstract

The in vivo activities of arginine and ornithine decarboxylases, key enzymes in the biosynthesis of putrescine and thus polyamines, were measured in three different cell lines of carrot (Daucus carota) during growth and somatic embryogenesis. The activities of these two enzymes differed in the different cell lines in the presence of various levels of auxin (2,4 dichlorophenoxy acetic acid), but was highest during periods of active cell division. During somatic embryo development, the activities of both enzymes were highest during globular stage formation. Thus, both enzymes were found to be active during growth and somatic embryogenesis and could contribute to polyamine biosynthesis.

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Natalia Loukanina and Trevor A. Thorpe "Arginine and ornithine decarboxylases in embryogenic and non-embryogenic carrot cell suspensions," In Vitro Cellular and Developmental Biology - Plant 44(1), 59-64, (19 September 2007). https://doi.org/10.1007/s11627-007-9080-3

Received: 7 September 2006; Accepted: 23 July 2007; Published: 19 September 2007

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