Enhanced numbers of multiple shoots were induced from shoot tip explants of cucumber. The effects of amino acids (leucine, isoleucine, methionine, threonine, and tryptophan) and polyamines (spermidine, spermine, and putrescine) along with benzyladenine (BA) on multiple shoot induction were investigated. A Murashige and Skoog (MS) medium containing a combination of BA (4.44 μM), leucine (88 μM), and spermidine (68 μM) induced the maximum number of shoots (36.6 shoots per explant) compared to BA (4.44 μM) alone or BA (4.44 μM) with leucine (88 μM). The regenerated shoots were elongated on the same medium. Elongated shoots were transferred to the MS medium fortified with BA (4.44 μM), leucine (88 μM), and putrescine (62 μM) for root induction. Rooted plants were hardened and successfully established in soil with a 90% survival rate.
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5 June 2008
Leucine and spermidine enhance shoot differentiation in cucumber (Cucumis sativus L.)
A. Vasudevan,
N. Selvaraj,
A. Ganapathi,
S. Kasthurirengan,
V. Ramesh Anbazhagan,
M. Manickavasagam,
C. W. Choi
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In Vitro Cellular and Developmental Biology - Plant
Vol. 44 • No. 4
July 2008
Vol. 44 • No. 4
July 2008
cucumber
Leucine
putrescine
root development
Shoot differentiation
Spermidine