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30 September 2021 Protein Electrophoresis of Serum and Heparinized Plasma in the Common Mynah (Acridotheres tristis)
Bahareh Aryakia, Mahdieh Zaeemi, Mohammad Heidarpour, Jamshid Razmyar
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Abstract

Although serum protein electrophoresis is a diagnostic tool available through many veterinary laboratories, there currently are no reference intervals for protein fractions in healthy common mynahs (Acridotheres tristis). Therefore, electrophoretic patterns of proteins in serum and heparinized plasma of the common mynah were evaluated. Blood specimens were collected from 55 healthy adult common mynahs of unknown age (26 males and 29 females). The serum total protein and protein fractions were measured using the biuret method followed by cellulose acetate electrophoresis (CAE). The serum level of albumin was compared with bromocresol green (BCG) dye-binding and CAE methods. Four protein fractions, including albumin and α, β, and γ globulins, were recorded in the electrophoretogram of serum specimens. Sex appeared to have no significant effect on the measured parameters. The serum BCG albumin fraction was significantly higher than the CAE albumin fraction (P = .01). Also, the comparison of total protein and protein fractions in serum and plasma specimens of 25 of the 55 birds sampled showed that total protein (Cohen index d = 0.66, P = .03), gamma globulin (d = 1.13, P = .00), and total globulin (d = 0.67, P = .00) in plasma samples were significantly higher than those in serum samples. The results of this study provide the specific reference intervals for total protein and protein fractions in common mynahs, which are essential for proper interpretation of laboratory results and also revealed that the albumin measurement by the BCG method yields unreliable results in common mynahs.

© 2021 by the Association of Avian Veterinarians
Bahareh Aryakia, Mahdieh Zaeemi, Mohammad Heidarpour, and Jamshid Razmyar "Protein Electrophoresis of Serum and Heparinized Plasma in the Common Mynah (Acridotheres tristis)," Journal of Avian Medicine and Surgery 35(3), 305-312, (30 September 2021). https://doi.org/10.1647/20-00025
Published: 30 September 2021
KEYWORDS
Acridotheres tristis
Avian
blood proteins
bromocresol green
cellulose acetate electrophoresis
common mynah
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