Liu, R.N.; Lin, Y.W.; Ye, X.D.; Wan, S.; Xu, G.D., and Li, Y.F., 2020. Mixed enzymes on sequential biofuel productivity in an anaerobic digestion system of molasses wastewater. In: Guido Aldana, P.A. and Kantamaneni, K. (eds.), Advances in Water Resources, Coastal Management, and Marine Science Technology. Journal of Coastal Research, Special Issue No. 104, pp. 497–503. Coconut Creek (Florida), ISSN 0749-0208.
In anaerobic system, the garbage could promote the biofuel production enzymes produced from PR consumer organic waste containing multi hydrolytic enzyme activity. A continuous stirred tank reactor (CSTR) was used to treat molasses wastewater for hydrogen and ethanol production in this study. To achieve maximum energy conversion in the anaerobic digestion system, six addition proportions of garbage enzymes (APE=0, 1:20, 1:15, 1:10, 1:5,1:4) were investigated. The result showed the maximum of volatile suspended solids was 29.34 g/L and chemical oxygen demand removal rate up to 90.18% respectively were obtained at optimum enzyme dose of APE=1:10. The hydrogen production rate(HPR) and ethanol production rate(ERP) were proportional to the APE at relative excess substrate concentration, and under ethanol fermentation, HPR and ERP reached maximum value of 0.67mol/d and 0.27 mol/d with the highest content of 49.56% and 41.73%, respectively. But the system production rate exhibited a non-linear correlation with the APE. This study demonstrated that enzymes acted synergistically with hydrogen producing microorganisms and could help maximize energy recovery efficiency.