How to translate text using browser tools
1 June 2017 Detection of Carbofuran Pesticide in Seawater by Using an Enzyme Biosensor
Zhuang Li, Song Qu, Lei Cui, Shuping Zhang
Author Affiliations +

Li, Z.; Qu, S.; Cui, L., and Zhang, S., 2017. Detection of carbofuran pesticide in seawater by using an enzyme biosensor. In: Zhi, Y. and Guido Aldana, P.A. (eds.), Sustainability of Water Resources and the Development of Coastal Environments: Select Proceedings from the 2016 International Conference on Water Resource and Environment (WRE2016). Journal of Coastal Research, Special Issue No. 80, pp. 1–5. Coconut Creek (Florida), ISSN 0749-0208.

With the development of agriculture in China and the large-scale use of pesticides, more and more pesticides enter the ocean through surface runoff and other ways. The threat to the water quality is becoming more and more serious. However, how to detect the pesticides quickly in the water becomes an important problem. Ground on acetylcholinesterase' (AChE) inhibition, the paper states the construction of an amperometric biosensor for carbamate insecticide carbofuran's highly sensitive detection. Using an enzyme biosensor ground on poly diallyldimethylammonium chloride (PDDA) and multi-walled carbon nanotubes (MWNTs) modified glassy carbon electrode (GCE), then immobilizing the Acetylcholinesterase (AChE) for modifying GCE with layer-by-layer (LBL) technique directly. Detect the inhibition of Aches with the amperometric i-t response. The pesticide concentration is correlated to the percentage inhibition of enzyme activity. Comparing with the enzyme biosensor {PDDA/MWNTs}5/ED/AChE/GCE we built previously with detection limit to be 10−11 g/L and recovery to be 99.8±5.7% to 10−8 g/L, nowadays, the new enzyme electrode shows a good linearity ranging from 10−12 g/L to 10−6 g/L between the −lg[carbofuran] and inhibition rates, and the detection limit is 10−12 g/L. The improvement of the electrode structure contributes significantly to the sensibility of detection for pesticide in seawater.

©Coastal Education and Research Foundation, Inc. 2017
Zhuang Li, Song Qu, Lei Cui, and Shuping Zhang "Detection of Carbofuran Pesticide in Seawater by Using an Enzyme Biosensor," Journal of Coastal Research 80(sp1), 1-5, (1 June 2017).
Received: 5 May 2016; Accepted: 1 September 2016; Published: 1 June 2017
layer-by-layer (LBL)
Get copyright permission
Back to Top