Methyl farnesoate (MF) was identified for the first time in the hemolymph of Nephrops norvegicus from Mediterranean deep-sea waters (around 500 m) by gas chromatography-mass spectometry and quantified by liquid chromatography. The MF was present in females as well as in males, although no clear-cut differences between sexes were observed. Levels of MF found in the hemolymph of intact animals were 0.5–1.0 ng/ml irrespective of the sex. Although the observations are still limited, females showed oscillations in MF that seem to parallel the vitellogenic cycle. Eyestalk ablation, in both males and females, caused a 10-fold increase in MF hemolymph levels.