The evaluation of potato germplasm for resistance to potato tuberworm, Phthorimaea operculella (Zeller) (Lepidoptera: Gelechiidae), is a valuable component of integrated pest management; however, few attempts have been made to identify natural genetic tuber resistance to tuberworm on potato germplasm. The objective of this study was to screen potato germplasm with potential tuberworm resistance for tuber resistance under field and laboratory conditions. Experiments were conducted over a 2-yr period at the Hermiston Agricultural Research and Extension Center, Hermiston, OR. Of 125 germplasm that were tested in 2006, q13 were selected for further screening in 2007. These germplasm were: A0008-1TE, A97066LB, NY123, PA00N10-5, PA99N2, PA99N82, Paciencia, Q174-2, Russet Burbank, Rubi, Ranger Russet, Spunta G2, and T88-4. Tuber resistance of potato germplasm was determined based on the number of mines per tuber and the number of live larvae. Tubers of transgenic clone Spunta G2 were resistant to tuberworm damage. All other germplasm tested in this study, including Russet Burbank and Ranger Russet, were susceptible to tuberworm in the field and laboratory experiments. Incorporation of host plant resistance to tuber penetration by larvae together with appropriate cultural practices including limiting exposure time of tubers in the field may provide the best management option in the future.