1 December 2009 Evaluation of Piperonyl Butoxide as a Deltamethrin Synergist for Pyrethroid-Resistant Bed Bugs
Alvaro Romero, Michael F. Potter, Kenneth F. Haynes
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An understanding of the mechanisms of insecticide resistance in the bed bug, Cimex lectularius L., has the potential to lead to new approaches for the control of resistant populations. We used the cytochrome P450 monooxygenase (P450) inhibitor piperonyl butoxide (PBO) to assess the role of P450s in deltamethrin resistance in three field-collected bed bug strains, LA-1, CIN-1 and WOR-1. In addition, we exposed two highly resistant strains, CIN-1 and WOR-1 (resistance ratio [RR] >2,500-fold), to dry residues of piperonyl butoxide-synergized pyrethroid formulations to determine the utility of synergism by PBO. Piperonyl butoxide synergized deltamethrin in all three strains, but its impact was variable. The synergistic ratio varied from 40 in CIN-1 to 176 in WOR-1. Because the resistance ratio for each strain after piperonyl butoxide treatment was 174 and 39, respectively, our results suggest that P450s have some involvement in deltamethrin resistance, but other resistance mechanisms must be involved as well. No significant synergistic effect of formulated deltamethrin was observed with the addition of synergized Pyrethrins or formulated piperonyl butoxide in the CIN-1 strain, but synergism occurred in the WOR-1 strain. Addition of PBO to pyrethroids is not a comprehensive solution to pyrethroid resistance because strains vary in both overall resistance level and the proportion of that resistance attributable to P450s.

© 2009 Entomological Society of America
Alvaro Romero, Michael F. Potter, and Kenneth F. Haynes "Evaluation of Piperonyl Butoxide as a Deltamethrin Synergist for Pyrethroid-Resistant Bed Bugs," Journal of Economic Entomology 102(6), 2310-2315, (1 December 2009). https://doi.org/10.1603/029.102.0637
Received: 9 July 2009; Accepted: 1 September 2009; Published: 1 December 2009

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Cimex lectularius
insecticide synergism
piperonyl butoxide
pyrethroid resistance
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