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1 August 2012 The Utility of Camptothecin as a Synergist of Bacillus thuringiensis var. kurstaki and Nucleopolyhedroviruses Against Trichoplusia ni and Spodoptera exigua
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Abstract

We studied the effect of combining microbial pesticides with camptothecin (CPT) on the mortality of two lepidopteran insects: Trichoplusia ni (Hübner) and Spodoptera exigua (Hübner). CPT is an alkaloid that is often used as an anticancer agent. Here, CPT was evaluated as a microbial pesticide synergist of Bacillus thuringiensis (Bt) and insect baculovirus. The toxicity of CPT and its synergistic effects on two microbial pesticides were studied using the diet overlay method. Bioassay results showed that CPT significantly enhances the toxicity of Bt variety kurstaki to S. exigua and T. ni. In addition, CPT strongly enhanced the infectivity of Autographa californica (Speyer) multinucleocapsid nucleopolyhedrovirus (AcMNPV) and S. exigua nucleopolyhedrovirus (SeMNPV). Using light microscopy, we found that CPT disrupts the peritrophic membrane of T. ni larvae and severely affects the structure of the midgut, resulting in an abnormal gut lumen morphology. We speculate that CPT increases toxicity by affecting the permeability of the peritrophic membrane.

© 2012 Entomological Society of America
Shifeng Sun, Zhongshan Cheng, Jing Fan, Xinghu Cheng, and Yi Pang "The Utility of Camptothecin as a Synergist of Bacillus thuringiensis var. kurstaki and Nucleopolyhedroviruses Against Trichoplusia ni and Spodoptera exigua," Journal of Economic Entomology 105(4), 1164-1170, (1 August 2012). https://doi.org/10.1603/EC12014
Received: 11 January 2012; Accepted: 1 March 2012; Published: 1 August 2012
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