Glenea cantor (F.) is a cerambycid species that attacks living trees of at least seven plant families in Asia, and has the potential to become an invasive pest. Here we investigated its reproductive biology in the laboratory to provide vital information for the development of pest control measures and preparation of pest risk analysis. Both sexes required a period of maturation feeding before mating, with the mean premating period of males (5.87 ± 1.68 d) being significantly longer than that of females (4.59 ± 2.34 d). This was a synovigenic species with the mean preoviposition and oviposition period being 13.50 ± 3.15 d and 49.46 ± 16.16 d, respectively. If no food was available after emergence, adult males died before reaching sexual maturation and females vanished before oviposition. Paired couples mated an average of 15.12 ± 7.02 times; 28- to 53-d-old beetles mated significantly more frequently than younger and older ones. The mean fecundity was 133.24 ± 9.67 eggs. The oviposition rate significantly increased in the first 5 wk of the oviposition period, peaking when females were 40- to 54-d-old, and then significantly decreased in the following weeks. Reproductive activities occurred almost exclusively during the day, with most ovipositions taking place in the morning and matings in the afternoon with some overlaps.