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6 June 2016 Diagnosis of Lobesia botrana (Lepidoptera: Tortricidae) Using Real-Time PCR
L. A. Ledezma, N. B. Barr, M. E. Epstein, T. M. Gilligan
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A real-time PCR assay is reported for identification of Lobesia botrana (Denis and Schiffermüller) collected in California. This assay multiplexes two independent TaqMan probe systems in a single reaction tube to reduce handling time and sample exposure to environmental contaminants. One probe system targets a segment of DNA located in the internal transcribed spacer region 2 (ITS2) that is present in the L. botrana genome but absent in native North American Tortricidae. The second probe system serves as a control for DNA quality by targeting a segment of the 18S rDNA gene that is conserved in L. botrana and all of the tested nontarget species. The assay successfully diagnosed 70 Lobesia botrana specimens and 95 nontarget specimens. No false-positive or false-negative results were observed supporting its application for identification of this pest in California.

Published by Oxford University Press on behalf of Entomological Society of America 2016. This work is written by US Government employees and is in the public domain in the United States.
L. A. Ledezma, N. B. Barr, M. E. Epstein, and T. M. Gilligan "Diagnosis of Lobesia botrana (Lepidoptera: Tortricidae) Using Real-Time PCR," Journal of Economic Entomology 109(4), 1957-1962, (6 June 2016).
Received: 16 February 2016; Accepted: 3 May 2016; Published: 6 June 2016

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