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25 April 2017 Identification and Expression Profiling of Odorant-Binding Proteins and Chemosensory Proteins of Daktulosphaira vitifoliae (Hemiptera: Phylloxeridae)
Jing-jing Zhao, Yue Zhang, Dong-sheng Fan, Ji-nian Feng
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Abstract

In insects, odorant-binding proteins (OBPs) and chemosensory proteins (CSPs) are primary peripheral olfactory proteins playing critical roles in odorant detection. In this study, we present the first identification of OBPs and CSPs from the transcriptome of grape phylloxera Daktulosphaira vitifoliae Fitch, an important pest that damages both roots and leaves of grapes. The OBPs contained six conserved cysteine residues and the CSPs contained four conserved cysteine residues in this insect. Phylogenetic analysis showed that most of the olfactory proteins were closely related to OBPs and CSPs from other aphids. However, DviOBP7 and DviCSP9 were different because they were classified into different independent branches, respectively. Real-time polymerase chain reaction (RT-PCR) was used to examine the tissue expression of these transcripts. DviOBP1, DviOBP6, and DviOBP7 were uniquely or primarily expressed in antennae and not in the body. DviOBP2 was more abundantly expressed in the body than in the antennae. The expression levels of OBPs and CSPs of phylloxera varied depending upon where they were expressed in different body tissues.

© The Authors 2017. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For Permissions, please email: journals.permissions@oup.com
Jing-jing Zhao, Yue Zhang, Dong-sheng Fan, and Ji-nian Feng "Identification and Expression Profiling of Odorant-Binding Proteins and Chemosensory Proteins of Daktulosphaira vitifoliae (Hemiptera: Phylloxeridae)," Journal of Economic Entomology 110(4), 1813-1820, (25 April 2017). https://doi.org/10.1093/jee/tox121
Received: 22 December 2016; Accepted: 30 March 2017; Published: 25 April 2017
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