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21 February 2018 PCR-Based Gut Content Analysis to Detect Predation of Eriococcus ironsidei (Hemiptera: Eriococcidae) by Coccinellidae Species in Macadamia Nut Orchards in Hawaii
Rosemary Gutierrez-Coarite, Nicole M. Yoneishi, Ishakh Pulakkatu-Thodi, Javier Mollinedo, Bernarda Calla, Mark G. Wright, Scott M. Geib
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Abstract

Macadamia felted coccid, Eriococcus ironsidei (Williams) (Hemiptera: Eriococcidae) was first found infesting macadamia trees in the island of Hawaii in 2005. Macadamia felted coccid infests all above-ground parts of trees to feed and reproduce.Their feeding activity distorts and stunts new growth which causes yellow spotting on older leaves, and when population densities become high, branch dieback occurs. Different predatory beetles have been observed in macadamia nut trees infested by E. ironsidei, the most abundant were Halmus chalybeus, Curinus coeruleus, Scymnodes lividigaster, Rhyzobius forestieri, and Sticholotis ruficeps.To verify predation of E. ironsidei by these beetles, a molecular assay was developed utilizing species-specific primers to determine presence in gut content of predators. Using these primers for PCR analysis, wild predator beetles were screened for the presence of E. ironsidei DNA. Analysis of beetles collected from macadamia orchards revealed predation by H. chalybeus, C. coeruleus, S. lividigaster, R. forestieri, and S. ruficeps on E. ironsidei.This study demonstrates that these beetles may play an important role in controlling the population of E. ironsidei, and these predators may be useful as biocontrol agents for E. ironsidei.

© The Author(s) 2018. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Rosemary Gutierrez-Coarite, Nicole M. Yoneishi, Ishakh Pulakkatu-Thodi, Javier Mollinedo, Bernarda Calla, Mark G. Wright, and Scott M. Geib "PCR-Based Gut Content Analysis to Detect Predation of Eriococcus ironsidei (Hemiptera: Eriococcidae) by Coccinellidae Species in Macadamia Nut Orchards in Hawaii," Journal of Economic Entomology 111(2), 885-891, (21 February 2018). https://doi.org/10.1093/jee/toy019
Received: 31 July 2017; Accepted: 10 January 2018; Published: 21 February 2018
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KEYWORDS
natural enemy
pest Management
predator
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