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20 March 2019 A Novel, Low-Cost Coconut Fiber Larval Diet for Mass Rearing Anastrepha (Diptera: Tephritidae)
Marysol Aceituno-Medina, Olivia Rincón-Betancurt, Rita Teresa Martínez-Salgado, Emilio Hernández
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Abstract

Mass rearing fruit flies of the genus Anastrepha for the sterile insect technique involves the use of artificial larval diets that commonly contain corn cob powder as a bulking agent. Corn cob powder varies in quality, and larval diets that contain it can be reaching at high temperatures (>34°C), which subsequently has a negative effect on pupal weight and adult survival. In addition, corn cob powder is susceptible to contamination by mycotoxins, which can inhibit larval development and cause high larval mortality. The objective of this work was to develop a low-cost artificial larval diet for mass rearing Anastrepha spp. fruit flies using coconut fiber, a nonconventional bulking agent, and to evaluate its effects on the quality parameters for A. ludens wild-type, A. ludensTap-7 GS-strain, A. obliqua, A. serpentina, and A. striata.The coconut fiber diet resulted in an increase in the larval and pupal weight of A. ludens Tap-7 GS-strain and A. obliqua, an increase in the larval weight of A. ludens wild-type, an increase in the flight ability of A. obliqua and A. serpentina and an increase in adult eclosion in A. ludensTap-7 GS-strain. The coconut fiber diet resulted in increased production and quality of the mass-reared flies, reduced the cost of the diet by 15 and 20% for A. ludens and A. obliqua, respectively, and led to further cost savings through reduced labor processes.

© The Author(s) 2019. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Marysol Aceituno-Medina, Olivia Rincón-Betancurt, Rita Teresa Martínez-Salgado, and Emilio Hernández "A Novel, Low-Cost Coconut Fiber Larval Diet for Mass Rearing Anastrepha (Diptera: Tephritidae)," Journal of Economic Entomology 112(3), 1112-1119, (20 March 2019). https://doi.org/10.1093/jee/toz044
Received: 16 August 2018; Accepted: 9 January 2019; Published: 20 March 2019
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KEYWORDS
bulking agent
generic larval diet
larval diet cost
mass rearing
sterile insect technique
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