Members of the highly polyphagous Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) species complex cause major crop damage by feeding and by transmitting plant viruses.The Middle East-Asia Minor 1 (MEAM1) of the B. tabaci complex is by far the most problematic whitefly affecting crops including cultivated watermelon (Citrullus lanatus; Cucurbitaceae: Cucurbitales). Watermelon cultivars share a narrow genetic base and are highly susceptible to whiteflies. We studied the potential of C. ecirrhosus, a perennial desert species that can be hybridized with C. lanatus, as a source of whitefly resistance for cultivated watermelon. The results of this study indicate that C. ecirrhosus offers resistance (although not total) against the MEAM1 B. tabaci based on, at least, antibiosis and antixenosis. Whitefly performance concerning developmental survival, body size attainment, and nonpreference were suppressed on C. ecirrhosus compared with the watermelon cultivar ‘Sugar Baby’. Also, our olfactometer results indicated that the adults were less attracted to leaf volatiles of C. ecirrhosus. Although there is a pungent odor associated with the leaves of C. ecirrhosus, the leaf volatiles had no toxic effect on adult whitefly survival as compared with cultivated watermelon. We also demonstrated that plants of C. ecirrhosus can be clonally propagated from vine cuttings of the parent plant. Using traditional breeding procedures, C. ecirrhosus was hybridized with C. lanatus and viable F₁ and F₂ seeds were produced. This is the first report of pest resistance in C. ecirrhosus. This wild species offers a source of resistance against whiteflies for the improvement of cultivated watermelon.