Western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), has developed resistance to transgenic corn that produces the insecticidal toxin Cry3Bb1 derived from the bacterium Bacillus thuringiensis (Bacillales: Bacillaceae) (Bt), with cross-resistance extending to corn with Bt toxins mCry3A and eCry3.1Ab. Additionally, some populations of western corn rootworm have evolved resistance to Cry34/35Ab1 corn. We conducted a 2-yr field and laboratory study that included three field locations: 1) Bt-susceptible population, 2) field with a recent history of Cry3Bb1 resistance, and 3) field with a long-term history of Cry3Bb1 resistance. The population with recently evolved Cry3Bb1 resistance showed resistance to Cry3Bb1 corn in both laboratory bioassays and field evaluations; by contrast, the population with a long-term history of Cry3Bb1 resistance showed resistance, in both laboratory and field experiments to Cry3Bb1 corn and corn with a pyramid of mCry3A plus eCry3.1Ab corn. Field-based evaluations also showed that the field population with a long-term history of Cry3Bb1 resistance imposed higher root injury to Cry3Bb1 corn and the pyramid of mCry3A plus eCry3.1Ab compared with the susceptible control. The results of this study are discussed in the context of developing strategies to manage western corn rootworm in areas where populations have evolved resistance to Cry3Bb1 corn.