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28 March 2022 Molecular Identification and Virulence of Four Strains of Entomopathogenic Fungi Against the Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)
Qian Lu, Peng Wang, Asad Ali, Lian-Sheng Zang
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The whitefly, Bemisia tabaci (Gennadius), is a key pest of many economically important crops grown in the field and in greenhouses throughout the world. Because entomopathogenic fungi (EPF) are potential biological control agents for B. tabaci, however, minimal research has been conducted on using fungal strains to control B. tabaci. In this study, four EPF strains were isolated and identified as Lecanicillium attenuatum (Zare & Gams) JL-003, Beauveria bassiana Balsamo (Vuillemin) JL-005, Lecanicillium longisporum (Petch) JL-006, and Akanthomyces lecanii (Zimmerman) JL-007, based on rDNA-ITS sequence analysis. In comparing the virulence of the four fungi against the different life stages (i.e., eggs, 1st-, 2nd-, 3rd-, 4th-instar nymphs, and adults) of B. tabaci the mortality of B. tabaci decreased and LT50 values increased as the conidia concentration decreased in a series of conidia concentrations (1 × 105, 106, 107, and 108 conidia/mL). The fungal strains L. attenuatum JL-003 (LC50: 1.31 × 106) and B. bassiana JL-005 (LC50: 0.92 × 106) were found to be more effective than L. longisporum JL-006 (LC50: 4.97 × 107) and A. lecanii JL-007 (LC50: 6.46 × 106). Fourth-instar nymphs, eggs, and adult stages of B. tabaci were less susceptible to all fungal strains compared to 1st-, 2nd-, and 3rd-instar nymphs. The virulence of L. attenuatum, which was tested for the first time on B. tabaci, was found to be more toxic to early-stage nymphs. Our data will be useful in biological control programs that are considering using EPF against B. tabaci.

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© The Author(s) 2022. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail:
Qian Lu, Peng Wang, Asad Ali, and Lian-Sheng Zang "Molecular Identification and Virulence of Four Strains of Entomopathogenic Fungi Against the Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)," Journal of Economic Entomology 115(3), 731-738, (28 March 2022).
Received: 16 October 2021; Accepted: 23 February 2022; Published: 28 March 2022

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Bemisia tabaci
biological control
entomopathogenic fungus
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