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29 May 2022 Genome-Wide Identification of M14 Family Metal Carboxypeptidases in Antheraea pernyi (Lepidoptera: Saturniidae)
Xian Zhang, Ping Zhao, Shanshan Li, Sanyuan Ma, Jie Du, Shimei Liang, Xinfeng Yang, Lunguang Yao, Jianping Duan
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Abstract

The M14 family metal carboxypeptidase genes play an important role in digestion and pathogenic infections in the gut of insects. However, the roles of these genes in Antheraea pernyi (Guérin-Méneville, 1855) remain to be analyzed. In the present study, we cloned a highly expressed M14 metal carboxypeptidase gene (ApMCP1) found in the gut and discovered that it contained a 1,194 bp open reading frame encoding a 397-amino acid protein with a predicted molecular weight of 45 kDa. Furthermore, 14 members of the M14 family metal carboxypeptidases (ApMCP1ApMCP14) were identified in the A. pernyi genome, with typical Zn_pept domains and two Zn-anchoring motifs, and were further classified into M14A, M14B, and M14D subfamilies. Expression analysis indicated that ApMCP1 and ApMCP9 were mainly expressed in the gut. Additionally, we observed that ApMCP1 and ApMCP9 displayed opposite expression patterns after starvation, highlighting their functional divergence during digestion. Following natural infection with baculovirus NPV, their expression was significantly upregulated in the gut of A. pernyi. Our results suggest that the M14 family metal carboxypeptidase genes are conservatively digestive enzymes and evolutionarily involved in exogenous pathogenic infections.

© The Author(s) 2022. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Xian Zhang, Ping Zhao, Shanshan Li, Sanyuan Ma, Jie Du, Shimei Liang, Xinfeng Yang, Lunguang Yao, and Jianping Duan "Genome-Wide Identification of M14 Family Metal Carboxypeptidases in Antheraea pernyi (Lepidoptera: Saturniidae)," Journal of Economic Entomology 115(4), 1285-1293, (29 May 2022). https://doi.org/10.1093/jee/toac081
Received: 20 January 2022; Accepted: 22 April 2022; Published: 29 May 2022
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KEYWORDS
Antheraea pernyi
digestion
pathogenic infection
the M14 family metal carboxypeptidase
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