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1 June 2000 Evaluation of Horn Flies (Diptera: Muscidae) from a Pyrethroid Susceptible Colony for General and Permethrin Esterase Activities
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Abstract

In this study we describe a nonradioactive single-fly microassay for permethrin hydrolysis. We used this assay with a microplate assay for general esterase activity to evaluate the permethrin hydrolyzing and general esterase activities of aging pyrethroid-susceptible male and female horn flies, Haematobia irritans (L.). We found substantial gender- and age-related differences regarding general esterase activity, permethrin sensitivity, and permethrin hydrolyzing activity within the colony. Extracts of female flies collected 48 h after receiving their first blood meal yielded significantly greater esterase activity than male extracts. Aging female flies were more tolerant of permethrin than were male flies. In addition, a positive correlation was found to exist between the general esterase activity of aging females and their ability to hydrolyze permethrin.

John H. Pruett, Delbert D. Oehler, Diane M. Kammlah, and Felix D. Guerrero "Evaluation of Horn Flies (Diptera: Muscidae) from a Pyrethroid Susceptible Colony for General and Permethrin Esterase Activities," Journal of Economic Entomology 93(3), 920-924, (1 June 2000). https://doi.org/10.1603/0022-0493-93.3.920
Received: 19 July 1999; Accepted: 1 January 2000; Published: 1 June 2000
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