Translator Disclaimer
1 October 2002 Isolation and Identification of an Esterase from a Mexican Strain of Boophilus microplus (Acari: Ixodidae)
Author Affiliations +
Abstract

A strain of Mexican Boophilus microplus (Cz) collected near Coatzacoalcos, Veracruz, Mexico, exhibits a moderate, but significant, level of permethrin resistance. Unlike other highly permethrin resistant strains, the Cz strain does not have a mutation within the sodium channel gene that results in target-site insensitivity. However, the Cz strain possesses a substantial increase in general and permethrin esterase activity relative to highly permethrin resistant and control strains suggesting the involvement of a metabolic esterase(s) in the expression of permethrin resistance. We report the isolation of a 62.8 kDa protein from Cz strain larvae that we think is the esterase previously reported as Cz EST9. In addition, internal amino acid sequence data obtained from the 62.8 kDa protein suggest that it is the gene product of a previously reported B. microplus carboxylesterase cDNA. We propose that the 62.8 kDa protein (Cz EST9) has permethrin hydrolytic activity and as a result plays an important role in Cz strain resistance to permethrin.

J. H. Pruett, F. D. Guerrero, and R. Hernandez "Isolation and Identification of an Esterase from a Mexican Strain of Boophilus microplus (Acari: Ixodidae)," Journal of Economic Entomology 95(5), 1001-1007, (1 October 2002). https://doi.org/10.1603/0022-0493-95.5.1001
Received: 16 October 2001; Accepted: 1 April 2002; Published: 1 October 2002
JOURNAL ARTICLE
7 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top