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1 February 2003 Comparison of Microcapsule Density with Various Apple Tissues and Formulations of Oriental Fruit Moth (Lepidoptera: Tortricidae) Sprayable Pheromone
Daniel E. Waldstein, L. J. Gut
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Abstract

The density of microcapsules was compared on ‘Golden Delicious’ mature and immature foliage, fruit, and 1-yr-old limbs after dip treatments with Phase III oriental fruit moth, Grapholita molesta (Busck), microencapsulated pheromone. Microcapsules were counted with a dissecting microscope at 50× magnification after apple tissues were treated with a two-step dye staining process to increase the visibility of the microcapsules. The number of Phase III microcapsules on 1-yr limbs treated with a field concentration was two- to threefold greater than the number of microcapsules on similarly treated fruit or foliage. Phase I, III, and V OFM MEC and Checkmate OFM-F formulations of microencapsulated pheromone also were compared to determine their abundance on mature apple foliage. The Phase V OFM MEC formulation had the highest density of microcapsules when mature foliage was treated at the field rate. The Phase I treatment had the greatest difference between upper and lower leaf surfaces with 18-fold greater microcapsule density on the upper surface. On mature apple leaves treated with Phase III MEC, the number of microcapsules/cm2 was two- to threefold greater on fields of view without the mid-vein than those that included the mid-vein. The cuticle structure and abundance of trichomes are two factors that may have contributed to differences in microcapsule density among plant tissue types, top and bottom leaf surfaces, and fields of view with and without the mid-vein.

Daniel E. Waldstein and L. J. Gut "Comparison of Microcapsule Density with Various Apple Tissues and Formulations of Oriental Fruit Moth (Lepidoptera: Tortricidae) Sprayable Pheromone," Journal of Economic Entomology 96(1), 58-63, (1 February 2003). https://doi.org/10.1603/0022-0493-96.1.58
Received: 1 March 2002; Accepted: 1 August 2002; Published: 1 February 2003
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KEYWORDS
apple
Grapholita molesta
mating disruption
microencapsulation
sprayable pheromone
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