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1 April 2003 Design and Evaluation of an Aerial Spray Trial with True Replicates to Test the Efficacy of Bacillus thuringiensis Insecticide in a Boreal Forest
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Abstract

A field trial using true replicates was conducted successfully in a boreal forest in 1996 to evaluate the efficacy of two aerially applied Bacillus thuringiensis formulations, ABG 6429 and ABG 6430. A complete randomized design with four replicates per treatment was chosen. Twelve to 15 balsam fir (Abies balsamea [L.] Mill.) per plot were randomly selected as sample trees. Interplot buffer zones, ≥ 200 m wide, adequately prevented cross contamination from sprays that were atomized with four rotary atomizers (volume median diameters ranging from 64.6 to 139.4 μm) and released ≈30 m above the ground. The B. thuringiensis formulations were not significantly different (P > 0.05) from each other in reducing spruce budworm (Choristoneura fumiferana [Clem.]) populations and protecting balsam trees from defoliation but both formulations were significantly more efficacious than the controls. The results suggest that true replicates are a feasible alternative to pseudoreplication in experimental forest aerial applications.

Beresford L. Cadogan and Roger D. Scharbach "Design and Evaluation of an Aerial Spray Trial with True Replicates to Test the Efficacy of Bacillus thuringiensis Insecticide in a Boreal Forest," Journal of Economic Entomology 96(2), 388-395, (1 April 2003). https://doi.org/10.1603/0022-0493-96.2.388
Received: 22 May 2002; Accepted: 1 October 2002; Published: 1 April 2003
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