Understanding local knowledge about wildlife, local uses, and local people's willingness to support conservation activities are crucial factors in formulating wildlife conservation strategies. We conducted a semi-structured questionnaire survey of 1017 people from 105 villages located in different ecological regions across all seven Provinces of Nepal. We performed generalized linear mixed modeling (GLMM) to investigate the key drivers influencing respondents' knowledge about pangolins, based on a questionnaire score. We identified provinces, ethnicity, occupation, gender, and age group as strong predictors influencing local ecological knowledge about pangolins. The respondents from provinces in the western part of Nepal represented significantly lower knowledge scores than the respondents from the eastern and central provinces. Similarly, respondents belonging to non-Indigenous groups, students, females, and people aged between 18–30 years had the lowest knowledge scores about pangolins. A range of uses and beliefs about pangolins were reported across Nepal, and 48% of respondents believed that pangolin scales and meat were used for traditional medicines. The majority of respondents (71.1%) were willing to support pangolin conservation in their local areas. Our study suggests that awareness programs, alternative livelihood, and income-generating trainings for local communities could be helpful in enhancing the long-term conservation of pangolins in Nepal. Increased efforts are needed in western Nepal and across the country with non-Indigenous communities, females, young people, and students. Thus, this study offers an important baseline to help design and execute effective community-based conservation actions and management decisions for pangolin conservation.