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30 May 2022 Molecular and Serological Detection of Leptospira spp. in Italian Tortoises (Testudo spp.)
Maria Luisa Marenzoni, Sara Bellucci, Valentina Stefanetti, Oriana Raffaele, Maria Lorenza Corbucci, Gianluca Deli, Erika Baldoni, Daniele Marini, Laura Vieceli, Marta Biaggini, Mario D'Incau, Patrizia Casagrande Proietti, Maria Pia Franciosini, Francesco Origgi, Claudia Corti, Trabalza-Marinucci Massimo
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Abstract

Leptospirosis is a worldwide zoonosis, characterized by a complex ecological cycle, including the environment, wild and domestic animals, and humans. It is also considered a water-borne infection. Although a solid body of data is available for more conventional hosts, limited information is available on neglected species such as reptiles, especially Testudinidae, both captive and free-ranging. Molecular and serological tests were performed to detect Leptospira spp. in Testudo spp. in Italy. Leptospiral DNA was detected using cloacal swabs collected from 116 individuals (54 free-ranging and 62 captive tortoises) by polymerase chain reaction (PCR). Unfortunately, because of the presence of PCR inhibitors, swabs from only 50 animals were eligible for analysis. A serological microagglutination test was performed on 121 Testudo spp. (7 free-ranging and 114 rescued tortoises). Sixteen individuals were tested using both assays, and the remainder were tested with one or the other. Ten out of 50 (20%) amplifiable cloacal swabs were positive for Leptospira DNA, the identity of which was confirmed by sequencing two amplicons. Two out of four repeatedly sampled animals only tested positive in the autumn. Seroprevalence was relatively low (0.8%, 1/121), at low titer (1:100). No positivity was recorded among free-ranging animals. Although these results do not provide a complete epidemiological snapshot of leptospirosis in chelonians, they suggest that tortoises are susceptible to pathogenic Leptospira. Further data are needed to determine the role of the captive environment as a possible predisposing factor and to assess the role of these reptiles in the ecology of leptospirosis.

Maria Luisa Marenzoni, Sara Bellucci, Valentina Stefanetti, Oriana Raffaele, Maria Lorenza Corbucci, Gianluca Deli, Erika Baldoni, Daniele Marini, Laura Vieceli, Marta Biaggini, Mario D'Incau, Patrizia Casagrande Proietti, Maria Pia Franciosini, Francesco Origgi, Claudia Corti, and Trabalza-Marinucci Massimo "Molecular and Serological Detection of Leptospira spp. in Italian Tortoises (Testudo spp.)," Journal of Herpetological Medicine and Surgery 32(2), 136-143, (30 May 2022). https://doi.org/10.5818/JHMS-D-21-00038
Published: 30 May 2022
JOURNAL ARTICLE
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KEYWORDS
Leptospira spp
MAT
PCR
Testudo
Testudo hermanni
Tortoises
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